A microplate fluorimetric assay for measuring dehalogenase activity

Marchesi, Julian Roberto ORCID: https://orcid.org/0000-0002-7994-5239 2003. A microplate fluorimetric assay for measuring dehalogenase activity. Journal of Microbiological Methods 55 (1) , pp. 325-329. 10.1016/S0167-7012(03)00132-5

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Abstract

A fluorimetric assay was developed to measure halide release from halogenated compounds being degraded by microbes. The method relies on the property of halides to quench the fluorescence of 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) by collision quenching. The assay shows a wide response to halide concentration (1–500 mM) and tolerates a wide pH range. Furthermore, it is simple to use, has the potential for automation and uses an inexpensive non-toxic reagent.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences Research Institutes & Centres > Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	Halide ions; Dehalogenation; Fluorimetric; SPQ; Microplate based.
Publisher:	Elsevier
ISSN:	0167-7012
Last Modified:	27 Oct 2022 08:35
URI:	https://orca.cardiff.ac.uk/id/eprint/62739

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