Inducible transgene expression in undifferentiated mouse embryonic stem cells and embryoid bodies

Nordin, N., Nathan, S., Li, Meng ORCID: https://orcid.org/0000-0002-4803-4643 and Mason, J. O. 2008. Inducible transgene expression in undifferentiated mouse embryonic stem cells and embryoid bodies. The Medical Journal of Malaysia 63 (Supp A) , pp. 59-60.

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Abstract

Obtaining pure population of neural cells from embryonic stem (ES) cells remains a challenge as little is known about the genes that govern embryonic stem cell differentiation. Using mouse ES cells, we aim to uncover the mechanisms that regulate neural differentiation of ES cells by focusing on roles played by Wnt family genes. Combining two techniques, Cre/loxP-based genetic recombination and ligand-dependent activation of Cre, we have generated transgenic ES cell lines that allow for the temporal control of expression and activity of Wnt gene (Wnt1-Ha) and Wnt antagonist (Dkk1). The ability of these cell lines in inducing the expression of transgene in undifferentiated ES cells and, more importantly, in differentiated derivatives of ES cells in vitro is evaluated.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine
Subjects:	Q Science > Q Science (General)
Publisher:	Malaysian Medical Association
ISSN:	0300-5283
Last Modified:	27 Oct 2022 08:41
URI:	https://orca.cardiff.ac.uk/id/eprint/63112

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