Morphological and functional changes of dissociated single pancreatic acinar cells: testing the suitability of the single cell as a model for exocytosis and calcium signaling

Park, Myoung Kyu, Lee, Misun and Petersen, Ole Holger ORCID: https://orcid.org/0000-0002-6998-0380 2004. Morphological and functional changes of dissociated single pancreatic acinar cells: testing the suitability of the single cell as a model for exocytosis and calcium signaling. Cell Calcium 35 (4) , pp. 367-379. 10.1016/j.ceca.2003.10.003

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Abstract

Isolated single pancreatic acinar cells have long been used as a model for studying many kinds of signaling processes due to their structural and functional polarities, but without significant validation. In this study, we examined the morphological and functional changes of dissociated single pancreatic acinar cells. Acutely isolated single cells showed a collapsed membrane potential and a much reduced secretion of zymogen granules in response to acetylcholine (ACh) stimulation, whereas clustered cells showed a much more negative membrane potential and potent exocytotic secretion. The isolated single cells became vertically flattened due to the loss of supporting adhesions with nearby cells, and the granule-attached luminal membrane was severely reduced versus that of clustered cells. However, polarized Ca2+ signals and mitochondrial localizations were relatively well preserved in the isolated single cells, in that Ca2+ release by ACh commenced at the indented luminal membrane. In clusters, the Ca2+ release site was closest to the lumen where more than three cells met or at the tips of conical regions of the luminal membrane. These findings suggest that the dissociated single pancreatic acinar cells preserve an intact Ca2+ signaling machinery but alter in shape and have impaired exocytotic functions and resting membrane potentials.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Systems Immunity Research Institute (SIURI)
Uncontrolled Keywords:	Pancreatic acinar cells; Calcium signaling; Exocytosis; Zymogen granules; Calcium wave; Luminal membrane; Morphology; Model; Acetylcholine
Publisher:	Elsevier
ISSN:	0143-4160
Last Modified:	27 Oct 2022 08:41
URI:	https://orca.cardiff.ac.uk/id/eprint/63138

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