Early membrane depressions in hepatocytes cultured on Primaria™ supports

Griffiths, Benjamin John Nathan and Evans, Peter J. 2001. Early membrane depressions in hepatocytes cultured on Primaria™ supports. Cell Biology International 25 (5) , pp. 489-494. 10.1006/cbir.2000.0623

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Abstract

The topography of spreading hepatocytes on positively charged Primaria™ plates was examined using scanning electron microscopy. The cells acquired a uniform morphology within 2h. The spreading was rapid and the surface of the cells showed early prominent depressions or dips. The hepatocytes had either one or two of these structures which corresponded to the frequency of mononuclear and binuclear cells, respectively. The nuclear origin of the dips was strengthened after 6h. They contained solid structures, whose number, size and shape were the same as nucleoli. The membrane dipping was independent of cell density and took place under conditions where phenotypic changes can occur. Kidney proximal tubule cells had no dips. Co-cultures of hepatocytes and kidney proximal tubule cells showed that the cell types behave differently.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	Hepatocytes; spreading; Primaria™; membrane dips.
Publisher:	Wiley
ISSN:	1065-6995
Last Modified:	28 Jun 2019 02:01
URI:	https://orca.cardiff.ac.uk/id/eprint/63348

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