Baines, D. L., MacGregor, G. G. and Kemp, Paul J.  ORCID: https://orcid.org/0000-0003-2773-973X
2001.
Fatty acid modulation and sequence identity of fetal guinea pig alveolar type II cell amiloride-sensitive Na+ channel.
Biochemical and Biophysical Research Communications
288
(3)
, pp. 727-735.
10.1006/bbrc.2001.5828
|
Abstract
Removal of fetal lung fluid at birth is crucial to survival. In vivo, a reversal in the direction of vectorial, amiloride-sensitive Na+ transport can be stimulated by ETYA, a nonmetabolizable analogue of the naturally occurring unsaturated fatty acid, arachidonate. Using the patch-clamp technique, fetal guinea pig alveolar type II pneumocyte single Na+ channel activity was robustly activated by 10 μM arachidonate, ETYA, oleate and stearate; this was unaffected by cyclooxygenase and 5′lipoxygenase inhibitors. The Na+ channel expressed in fetal guinea pig alveolar epithelial type II pneumocytes has biophysical properties compatible with species-specific coexpression of a novel variant of αENaC with βENaC. γENaC is either not expressed in this tissue or shares very little homology with the rat and human γ subunit. Thus, dramatic stimulation of this channel by arachidonate explains the in vivo observation of gestation-dependent reversal of fetal transepithelial driving force and may, therefore, be of physiological significance during the transition to breathing air at birth.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences |
| Publisher: | Elsevier |
| ISSN: | 0006-291X |
| Last Modified: | 27 Oct 2022 08:48 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/63421 |
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