Cytokine-mediated differential regulation of macrophage activator protein-1 genes

Tengku-Muhammad, Tengku S., Hughes, Timothy R, Foka, Pelagia, Cryer, Anthony and Ramji, Dipak Purshottam ORCID: https://orcid.org/0000-0002-6419-5578 2000. Cytokine-mediated differential regulation of macrophage activator protein-1 genes. Cytokine 12 (6) , pp. 720-726. 10.1006/cyto.1999.0620

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Abstract

The regulation of macrophage activator protein-1 (AP-1) gene expression by LPS and cytokines is of potentially crucial importance in the pathogenesis of several diseases. The action of LPS and four cytokines on AP-1 gene expression in the murine macrophage J774.2 cell line was, therefore, studied. Exposure of the cells to IL-6 produced no changes in the mRNA levels of all AP-1 members studied. In contrast, the expression of JunB, c-jun and c-fos, but not JunD, was increased by LPS, TNF-α, IFN-γ and IL-1, albeit with different kinetics and magnitude of induction. Electrophoretic mobility shift assays showed a close correlation between the expression of the AP-1 genes and the functional AP-1 DNA binding activity and, additionally, demonstrated the participation of heterodimeric interactions between the different members. These studies provide insights into the potential mechanisms that may be involved in the mediator-specific modulation of AP-1 regulated macrophage gene expression.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	Activator protein 1; cytokines; J774.2 cells; lipopolysaccharide; gene expression.
Publisher:	Elsevier
ISSN:	1043-4666
Last Modified:	27 Oct 2022 08:48
URI:	https://orca.cardiff.ac.uk/id/eprint/63453

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