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Cytokine-mediated differential regulation of macrophage activator protein-1 genes

Tengku-Muhammad, Tengku S., Hughes, Timothy R, Foka, Pelagia, Cryer, Anthony and Ramji, Dipak Purshottam ORCID: 2000. Cytokine-mediated differential regulation of macrophage activator protein-1 genes. Cytokine 12 (6) , pp. 720-726. 10.1006/cyto.1999.0620

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The regulation of macrophage activator protein-1 (AP-1) gene expression by LPS and cytokines is of potentially crucial importance in the pathogenesis of several diseases. The action of LPS and four cytokines on AP-1 gene expression in the murine macrophage J774.2 cell line was, therefore, studied. Exposure of the cells to IL-6 produced no changes in the mRNA levels of all AP-1 members studied. In contrast, the expression of JunB, c-jun and c-fos, but not JunD, was increased by LPS, TNF-α, IFN-γ and IL-1, albeit with different kinetics and magnitude of induction. Electrophoretic mobility shift assays showed a close correlation between the expression of the AP-1 genes and the functional AP-1 DNA binding activity and, additionally, demonstrated the participation of heterodimeric interactions between the different members. These studies provide insights into the potential mechanisms that may be involved in the mediator-specific modulation of AP-1 regulated macrophage gene expression.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Activator protein 1; cytokines; J774.2 cells; lipopolysaccharide; gene expression.
Publisher: Elsevier
ISSN: 1043-4666
Last Modified: 27 Oct 2022 08:48

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