Localisation of DNA topoisomerase II alpha in mouse erythroleukemia cells

Ivanova, E. C., Donev, Rossen Mintchev and Djondjurov, L. P. 1999. Localisation of DNA topoisomerase II alpha in mouse erythroleukemia cells. Molecules and Cells 9 (3) , pp. 309-313.

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Abstract

The presence of DNA topoisomerase II alpha was investigated in interphase and metaphase mouse erythroleukemia (MEL) Friend-S cells, and in extracted with 25 mM lithium diiodosalicylate buffer (Lis) nuclei using indirect immunofluorescence. The results showed that DNA topoisomerase II alpha is localised in the nuclei. In the metaphase cells, we found high concentrations of this enzyme in the mitotic chromosomes. Our results support the idea of the accumulation of DNA topoisomerase II alpha at the end of the cell cycle. The extractions of nuclei with 25 mM Lis led to the complete depletion of DNA topoisomerase II alpha from the residual nuclear matrix. Using a high dilution of the first antibody, we established that the high level of heterochromatin compactisation in the interphase nuclei is caused by the high concentration of DNA topoisomerase II alpha.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Animals, Antigens, Neoplasm, Cell Nucleus/enzymology, DNA Topoisomerases, Type II*/metabolism, DNA-Binding Proteins, Fluorescent Antibody Technique, Indirect, Friend murine leukemia virus, Heterochromatin/enzymology, Interphase, Isoenzymes/metabolism, Leukemia, Erythroblastic, Acute/enzymology, Leukemia, Erythroblastic, Acute/pathology, Metaphase, Mice, Tumor Cells, Cultured
Publisher:	Springer Verlag
ISSN:	1016-8478
Last Modified:	19 Mar 2016 23:45
URI:	https://orca.cardiff.ac.uk/id/eprint/63800

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