Extraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min

Aslan, K., Previte, M. J. R., Zhang, Y. X., Gallagher, T., Baillie, Les ORCID: https://orcid.org/0000-0002-8186-223X and Geddes, C. D. 2008. Extraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min. Analytical Chemistry (including News & Features) 80 (11) , pp. 4125-4132. 10.1021/ac800519r

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Abstract

The use of a combination of low-cost technologies to both extract and detect anthrax DNA from spores and vegetative cells in two steps within 1 min is described. In a cavity, microwave energy is highly focused using thin-film aluminum “bow-tie” structures, to extract DNA from whole spores within 20 s. The detection of the released DNA, from less than 1000 vegetative cells, without additional preprocessing steps is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. The new platform technology presented here is a highly attractive alternative method for DNA extraction and the fast detection of Gram-positive bacteria and potentially other pathogenic species and cells as well.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Publisher:	American Chemical Society
ISSN:	0003-2700
Last Modified:	17 Oct 2022 09:58
URI:	https://orca.cardiff.ac.uk/id/eprint/6414

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