Potassium channels in hippocampal neurones are absent in a transgenic but not in a chemical model of Alzheimer's disease

Coles, Barbara, Wilton, L. A. K., Good, Mark Andrew ORCID: https://orcid.org/0000-0002-1824-1203, Chapman, P. F. and Wann, Kenneth Taylor 2008. Potassium channels in hippocampal neurones are absent in a transgenic but not in a chemical model of Alzheimer's disease. Brain Research 1190 , pp. 1-14. 10.1016/j.brainres.2007.10.071

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Abstract

We have investigated using single channel patch-clamp methods potassium channel prevalence in hippocampal neurones from two animal models of AD. Experiments have been carried out on transgenic mice (Tg2576) carrying the Swedish mutation (K670N/M671L) and rats receiving ventricular infusions of okadaic acid. In cell-attached patches from hippocampal neurones from the Tg2576 and control littermate mice there were three principal unitary conductance — 22 pS, 111 pS and 178 pS. The two channels of intermediate and large conductance were voltage-dependent, highly active in cell-attached patches, activity decreasing markedly on hyperpolarisation. The large conductance channel was sensitive to TEA, iberiotoxin, was activated in excised inside-out patches by Ca 2+i and is the type I maxi-K+ channel. Significantly, there was a reduction in the prevalence of a TEA-sensitive 113 pS channel in neurones from TG2576 mice with a corresponding increase in prevalence of the maxi-K+ channel. There was no difference in the characteristics of maxi-K+ between patches in neurones from the transgenic and littermate controls. In the rat model single channel analysis was performed on hippocampal neurons from three groups of animals i.e. non-operated, and these receiving an infusion of vehicle or vehicle with okadaic acid. Three principal unitary conductances of around 18 pS, 118 pS and 185 pS were also observed in cell-attached recordings from these three groups. The intermediate and high conductance channels were blocked by TEA or 4-AP or 140 mM RbCl. There were no statistically significant differences in the channel prevalence or channel density between the control and test groups.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Psychology Pharmacy Biosciences
Subjects:	Q Science > QP Physiology R Medicine > R Medicine (General) R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Uncontrolled Keywords:	Hippocampus; Alzheimer's disease; Potassium channel; Transgenic; Phosphorylation
Publisher:	Elsevier
ISSN:	0006-8993
Last Modified:	17 Oct 2022 09:59
URI:	https://orca.cardiff.ac.uk/id/eprint/6477

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