Surface roughness mediated adhesion forces between borosilicate glass and gram-positive bacteria

Preedy, Emily Callard, Perni, Stefano, Nipic, Damijan, Bohinc, Klemen and Prokopovich, Polina ORCID: https://orcid.org/0000-0002-5700-9570 2014. Surface roughness mediated adhesion forces between borosilicate glass and gram-positive bacteria. Langmuir 30 (31) , pp. 9466-9476. 10.1021/la501711t

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Abstract

It is well-known that a number of surface characteristics affect the extent of adhesion between two adjacent materials. One of such parameters is the surface roughness as surface asperities at the nanoscale level govern the overall adhesive forces. For example, the extent of bacterial adhesion is determined by the surface topography; also, once a bacteria colonizes a surface, proliferation of that species will take place and a biofilm may form, increasing the resistance of bacterial cells to removal. In this study, borosilicate glass was employed with varying surface roughness and coated with bovine serum albumin (BSA) in order to replicate the protein layer that covers orthopedic devices on implantation. As roughness is a scale-dependent process, relevant scan areas were analyzed using atomic force microscope (AFM) to determine Ra; furthermore, appropriate bacterial species were attached to the tip to measure the adhesion forces between cells and substrates. The bacterial species chosen (Staphylococci and Streptococci) are common pathogens associated with a number of implant related infections that are detrimental to the biomedical devices and patients. Correlation between adhesion forces and surface roughness (Ra) was generally better when the surface roughness was measured through scanned areas with size (2 × 2 μm) comparable to bacteria cells. Furthermore, the BSA coating altered the surface roughness without correlation with the initial values of such parameter; therefore, better correlations were found between adhesion forces and BSA-coated surfaces when actual surface roughness was used instead of the initial (nominal) values. It was also found that BSA induced a more hydrophilic and electron donor characteristic to the surfaces; in agreement with increasing adhesion forces of hydrophilic bacteria (as determined through microbial adhesion to solvents test) on BSA-coated substrates.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Publisher:	ACS Publications
ISSN:	0743-7463
Funders:	ARUK
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	14 July 2014
Last Modified:	04 May 2023 10:25
URI:	https://orca.cardiff.ac.uk/id/eprint/64940

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