Vasoconstrictor agonists activate G-protein-dependent receptor-operated calcium channels in pig aortic microsomes

Blayney, Lynda Mary, Gapper, Peter William James and Newby, A. C. 1992. Vasoconstrictor agonists activate G-protein-dependent receptor-operated calcium channels in pig aortic microsomes. Biochemical Journal 282 (1) , pp. 81-84.

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Abstract

Receptor-operated Ca2+ channels were characterized by their ability to decrease steady-state ATP-dependent Ca2+ accumulation into pig aortic microsomes. The vasoconstrictor agents noradrenaline, angiotensin II and adenosine 5'-[alpha beta-methylene]triphosphate (pp[CH2]pA) all decreased Ca2+ accumulation only when sonicated into vesicles (to allow access to receptor sites) and in the presence of guanosine 5'-[beta gamma-imido]triphosphate to activate transducing G-proteins. The effect of noradrenaline was inhibited by the alpha 2 antagonist yohimbine, but not by the alpha 1 antagonist prazosin. The effect of none of the agonists was reversed by diltiazem. SK&F 96365 (an inhibitor of receptor-mediated Ca2+ influx into intact cells) reversed the effect of noradrenaline, but not that of pp[CH2]pA, which suggests that at least two receptor-operated channels may be present in this preparation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Portland Press
ISSN:	0264-6021
Related URLs:	http://www.ncbi.nlm.nih.gov/pubmed/13472...
Last Modified:	31 Jan 2020 04:19
URI:	https://orca.cardiff.ac.uk/id/eprint/65326

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