Aromatase activity in genital skin fibroblasts from normal and androgen-insensitive individuals

Stillman, S. C., Evans, Bronwen Alice James ORCID: https://orcid.org/0000-0002-3082-1008 and Hughes, I. A. 1990. Aromatase activity in genital skin fibroblasts from normal and androgen-insensitive individuals. Journal of Endocrinology 127 (1) , pp. 177-183. 10.1677/joe.0.1270177

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Abstract

Human genital skin fibroblasts were used to study aromatase activity by analysing the [3H]H2O released as [1β-3H]androstenedione is converted to oestrone. 4-Hydroxyandrostenedione was shown to be a competitive inhibitor of this aromatase activity, the concentration of inhibitor producing 50% inhibition being 1·29 nmol/l. Dexamethasone stimulated the enzyme complex in a dose-dependent manner with half-maximal stimulation at 11·5 nmol/l. A peak of induction occurred after 16 h of preincubation. Measurement of aromatase activity in normal cell strains provided a normal range for the Michaelis–Menten constant (Km) and the maximum velocity (Vmax) of 6·72±0·54 nmol/l and 215·3±33·9 fmol/mg protein per h (means ± s.e.m., n = 20) respectively. Km values obtained for partial and complete androgen insensitivity syndrome (PAIS and CAIS) patient cell strains were all within the normal range. The mean Vmax±s.e.m. in cell strains from patients with PAIS (n = 13) and CAIS (n = 11) were 127·4±19·2 and 54·8±19·3 fmol/mg protein per h respectively. Vmax values for patients with CAIS were significantly (P < 0·001) lower than normal subjects. This suggests that aromatase expression in genital skin fibroblasts is androgen-dependent.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Society for Endocrinology
ISSN:	0022-0795
Last Modified:	27 Oct 2022 09:21
URI:	https://orca.cardiff.ac.uk/id/eprint/65357

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