Glycosyl transferase activities are associated with the surface membrane in adult Schistosoma mansoni

Simpson, A. J., Rumjanek, F. D., Payares, G. and Evans, William Howard 1981. Glycosyl transferase activities are associated with the surface membrane in adult Schistosoma mansoni. Molecular and Biochemical Parasitology 4 (1-2) , pp. 107-115. 10.1016/0166-6851(81)90033-5

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Abstract

Incubation of live adult Schistosoma mansoni in a variety of media released tegumental material containing membrane bound alkaline phosphatase, mannosyl transferase and galactosyl transferase activities. Centrifugation of the tegumental material released by incubation of worms in phosphate-buffered saline in sucrose density gradients yielded a pellet and four fractions, two of which consisted mainly of surface membranes. The distribution of the enzymes in the gradient, espeically in the two surface membrane-containing subfractions was similar. Application of the "digitonin shift" technique showed that the membranes containing the enzyme reactivities were moved to an equal extent into a denser part of the sucrose gradient. Thus the enzymes are located on the same or similar cholesterol-containing membranes. It is concluded that the transferases, like the alkaline phosphatases, are located in the surface membranes of S. mansoni and the consequences of this location for the host-parasite interaction are discussed.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Publisher:	Elsevier
ISSN:	0166-6851
Last Modified:	17 Mar 2021 02:53
URI:	https://orca.cardiff.ac.uk/id/eprint/66152

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