Dechorionation of medaka embryos and cell transplantation for the generation of chimeras

Porazinski, Sean, Wang, Huijia and Furutani-Seiki, Makoto 2010. Dechorionation of medaka embryos and cell transplantation for the generation of chimeras. Journal of Visualized Experiments (46) , e2055. 10.3791/2055

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Abstract

Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
ISSN:	1940-087X
Last Modified:	01 Feb 2017 04:51
URI:	https://orca.cardiff.ac.uk/id/eprint/66555

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