Phospholipid, cholesterol, polypeptide and glycoprotein composition of hepatic endosome subfractions

Evans, William Howard and Hardison, W. G. 1985. Phospholipid, cholesterol, polypeptide and glycoprotein composition of hepatic endosome subfractions. Biochemical Journal -London- 232 (1) , pp. 33-36.

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Abstract

The composition of hepatic endosome subfractions was compared directly with that of plasma membranes and Golgi-apparatus fractions. The neutral lipid and phospholipid composition of two endosome subfractions separated on Nycodenz gradients from a parent endosome fraction was similar to that of plasma membranes. The phospholipid/cholesterol ratios and the sphingomyelin contents were high, as in plasma membranes. However, the phosphatidylserine content was low. Endosomal subfractions contained a simpler polypeptide profile than did plasma membranes. However, a large number of glycoproteins were common to both fractions. Two endosome-specific glycoproteins, of Mr 59 000 and 38 000, were identified. Sialic acid was present at concentrations higher than in plasma membranes. The results indicate that endosomal membranes have a similar composition to plasma membranes that probably reflects their functional interaction during endocytosis and receptor recycling.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Publisher:	Portland Press
ISSN:	0264-6021
Related URLs:	http://www.biochemj.org/bj/default.htm
Last Modified:	17 Mar 2021 02:58
URI:	https://orca.cardiff.ac.uk/id/eprint/66947

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