Molecular and insecticidal characterization of a novel cry-related protein from 'Bacillus Thuringiensis' toxic against 'Myzus persicae'

Palma, Leopoldo, Muñoz, Delia, Berry, Colin ORCID: https://orcid.org/0000-0002-9943-548X, Murillo, Jesús, de Escudero, Iñigo and Caballero, Primitivo 2014. Molecular and insecticidal characterization of a novel cry-related protein from 'Bacillus Thuringiensis' toxic against 'Myzus persicae'. Toxins 6 (11) , pp. 3144-3156. 10.3390/toxins6113144

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Abstract

This study describes the insecticidal activity of a novel Bacillus thuringiensis Cry-related protein with a deduced 799 amino acid sequence (~89 kDa) and ~19% pairwise identity to the 95-kDa-aphidicidal protein (sequence number 204) from patent US 8318900 and ~40% pairwise identity to the cancer cell killing Cry proteins (parasporins Cry41Ab1 and Cry41Aa1), respectively. This novel Cry-related protein contained the five conserved amino acid blocks and the three conserved domains commonly found in 3-domain Cry proteins. The protein exhibited toxic activity against the green peach aphid, Myzus persicae (Sulzer) (Homoptera: Aphididae) with the lowest mean lethal concentration (LC50 = 32.7 μg/mL) reported to date for a given Cry protein and this insect species, whereas it had no lethal toxicity against the Lepidoptera of the family Noctuidae Helicoverpa armigera (Hübner), Mamestra brassicae (L.), Spodoptera exigua (Hübner), S. frugiperda (J.E. Smith) and S. littoralis (Boisduval), at concentrations as high as ~3.5 μg/cm2. This novel Cry-related protein may become a promising environmentally friendly tool for the biological control of M. persicae and possibly also for other sap sucking insect pests.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords:	Bacillus thuringiensis; δ-endotoxins; Cry-related protein; parasporins; green peach aphid; Myzus persicae
Publisher:	Molecular Diversity Preservation International
ISSN:	2072-6651
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	28 October 2014
Last Modified:	23 May 2023 21:59
URI:	https://orca.cardiff.ac.uk/id/eprint/67029

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