Contribution of cell culture additives to the two-dimensional protein patterns of mouse macrophages

Miller, Ingrid, Radwan, Marta, Strobl, Birgit, Müller, Mathias and Gemeiner, Manfred 2006. Contribution of cell culture additives to the two-dimensional protein patterns of mouse macrophages. Electrophoresis 27 (8) , pp. 1626-1629. 10.1002/elps.200500744

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Abstract

Low levels of fetal calf serum (FCS), used as protein supplement in cell culture medium, were traced in preparations of primary murine macrophages (bone-marrow-derived macrophages (BMM) and peritoneal macrophages (PM)). Main components of this common additive were mapped in 2-DE by means of differential image gel electrophoresis and immunoblotting. Additional washing steps in cell preparation helped to decrease the levels of the four highest abundance foetal serum proteins (serum albumin (SA), α1-fetoprotein (AFP), α1-antitrypsin (α1AT) and transferrin (Tf)) to less than 1% of total protein. Macrophage spot pattern was recorded in parallel and showed little variation. Results presented are supposed to be of general interest for cell preparations with similar background.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	Fetal calf serum; Immunoblotting; Macrophage; Mouse; Two-dimensional electrophoresis.
Publisher:	Wiley
ISSN:	0173-0835
Last Modified:	12 Oct 2016 03:19
URI:	https://orca.cardiff.ac.uk/id/eprint/67820

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