Moseley, Ryan  ORCID: https://orcid.org/0000-0002-2812-6735, Waddington, Rachel J. ORCID: https://orcid.org/0000-0001-5878-1434, Sloan, Alastair James ORCID: https://orcid.org/0000-0002-1791-0903, Smith, Anthony J., Hall, R.C. and Embery, Graham
2003.
The influence of fluoride exposure on dentin mineralization using an in vitro organ culture model.
Calcified Tissue International
73
(5)
, pp. 470-475.
10.1007/s00223-003-0022-8
|
Abstract
This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing the apparent changes in mineral composition.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Dentistry |
| Subjects: | R Medicine > RK Dentistry |
| Uncontrolled Keywords: | Dentin ; Mineralization ; Fluoride ; Odontoblasts |
| ISSN: | 14320827 |
| Last Modified: | 17 Oct 2022 08:39 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/682 |
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