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The influence of fluoride exposure on dentin mineralization using an in vitro organ culture model

Moseley, Ryan ORCID:, Waddington, Rachel J. ORCID:, Sloan, Alastair James ORCID:, Smith, Anthony J., Hall, R.C. and Embery, Graham 2003. The influence of fluoride exposure on dentin mineralization using an in vitro organ culture model. Calcified Tissue International 73 (5) , pp. 470-475. 10.1007/s00223-003-0022-8

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This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing the apparent changes in mineral composition.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Dentin ; Mineralization ; Fluoride ; Odontoblasts
ISSN: 14320827
Last Modified: 17 Oct 2022 08:39

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