Dhopatkar, A.A., Sloan, Alastair James  ORCID: https://orcid.org/0000-0002-1791-0903, Rock, W.P., Cooper, P.R. and Smith, Anthony J.
2005.
A novel in vitro culture model to investigate the reaction of the dentine-pulp complex to orthodontic force.
Journal of Orthodontics
32
(2)
, pp. 122-132.
10.1179/146531205225020979
|
Abstract
Objective: To develop a novel mandible slice organ culture model to investigate the effects of externally applied force on the dentine–pulp complex. Design: In vitro organ culture. Setting: School of Dentistry, Birmingham, UK. Materials and methods: Transverse 2 mm thick sections were cut from the mandibles of five 28-day-old male Wistar rats. Serial sections were used for control and test pairs. Springs made from 0.016-inch and 0.019 x 0.025-inch stainless steel wires were used to apply a 50 g tensile or compressive force, respectively, to test specimens. Control and test specimens were cultured for 5 days in a humidified incubator with 5% CO2 at 37°C and processed for routine histological investigation. Nine more rats were used to provide control and compression test pairs where the pulps were extirpated after 3 days culture and total RNA isolated for gene expression analysis by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Histology showed the dental and supporting tissues maintained a healthy appearance in the control cultures after culture. Histomorphometric analysis revealed a 20–27% increase in pulp fibroblast density in test specimens compared with controls. Gene expression analyses revealed up-regulation in the test groups of PCNA, c-Myc, Collagen 1, TGF-ß1 and alkaline phosphatase, whilst expression of osteocalcin was reduced. Conclusions: The results demonstrated that the present organ culture technique provides a valuable in vitro experimental model for studying the effects of externally applied forces. These forces stimulated a cellular response in the pulp chamber characterized by altered gene expression and proliferation of fibroblasts; the latter being unaffected by the nature of the force in terms of compression or tension.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Dentistry |
| Subjects: | R Medicine > RK Dentistry |
| Uncontrolled Keywords: | Organ culture ; orthodontic force ; dentine-pulp complex |
| Additional Information: | "British Orthodontic Society, Chapman Prize Winner 2003" |
| ISSN: | 14653133 |
| Last Modified: | 17 Oct 2022 08:39 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/689 |
Citation Data
Cited 26 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Altmetric
Altmetric