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Identification of a type IX collagen interaction with fibronectin: a molecular bridge in articular cartilage?

Parsons, Philippa, Gilbert, Sophie Jane, Vaughan-Thomas, Anne, Sorrell, David A., Notman, Rebecca, Bishop, Mark, Mason, Deborah Jane and Duance, Victor Colin 2011. Identification of a type IX collagen interaction with fibronectin: a molecular bridge in articular cartilage? The Journal of Biological Chemistry 286 (40) , pp. 34986-34997. 10.1074/jbc.M111.238188

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Type IX collagen is covalently bound to the surface of type II collagen fibrils within the cartilage extracellular matrix. The N-terminal, globular noncollagenous domain (NC4) of the α1(IX) chain protrudes away from the surface of the fibrils into the surrounding matrix and is available for molecular interactions. To define these interactions, we used the NC4 domain in a yeast two-hybrid screen of a human chondrocyte cDNA library. 73% of the interacting clones encoded fibronectin. The interaction was confirmed using in vitro immunoprecipitation and was further characterized by surface plasmon resonance. Using whole and pepsin-derived preparations of type IX collagen, the interaction was shown to be specific for the NC4 domain with no interaction with the triple helical collagenous domains. The interaction was shown to be of high affinity with nanomolar Kd values. Analysis of the fibronectin-interacting clones indicates that the constant domain is the likely site of interaction. Type IX collagen and fibronectin were shown to co-localize in cartilage. This novel interaction between the NC4 domain of type IX collagen and fibronectin may represent an in vivo interaction in cartilage that could contribute to the matrix integrity of the tissue.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Collagen, Connective Tissue, Extracellular Matrix, Fibronectin, Protein-Protein Interactions, NC4, Type IX Collagen, Articular Cartilage.
Publisher: American Society for Biochemistry and Molecular Biology
ISSN: 0021-9258
Last Modified: 02 May 2019 11:06

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