Ryanodine receptor regulation by intramolecular interaction between cytoplasmic and transmembrane domains

George, Christopher, Jundi, Hala, Thomas, Nia Lowri ORCID: https://orcid.org/0000-0001-8822-8576, Scoote, Mark, Walters, Nicola, Williams, Alan John and Lai, Francis Anthony ORCID: https://orcid.org/0000-0003-2852-8547 2004. Ryanodine receptor regulation by intramolecular interaction between cytoplasmic and transmembrane domains. Molecular biology of the cell 15 (6) , pp. 2627-2638. 10.1091/mbc.E03-09-0688

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Abstract

Ryanodine receptors (RyR) function as Ca2+ channels that regulate Ca2+ release from intracellular stores to control a diverse array of cellular processes. The massive cytoplasmic domain of RyR is believed to be responsible for regulating channel function. We investigated interaction between the transmembrane Ca2+-releasing pore and a panel of cytoplasmic domains of the human cardiac RyR in living cells. Expression of eGFP-tagged RyR constructs encoding distinct transmembrane topological models profoundly altered intracellular Ca2+ handling and was refractory to modulation by ryanodine, FKBP12.6 and caffeine. The impact of coexpressing dsRed-tagged cytoplasmic domains of RyR2 on intracellular Ca2+ phenotype was assessed using confocal microscopy coupled with parallel determination of in situ protein: protein interaction using fluorescence resonance energy transfer (FRET). Dynamic interactions between RyR cytoplasmic and transmembrane domains were mediated by amino acids 3722-4610 (Interacting or "I"-domain) which critically modulated intracellular Ca2+ handling and restored RyR sensitivity to caffeine activation. These results provide compelling evidence that specific interaction between cytoplasmic and transmembrane domains is an important mechanism in the intrinsic modulation of RyR Ca2+ release channels.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	Publisher’s copyright requirements: “Authors are permitted to post the MBoC PDF of their articles (and/or supplemental material) on their personal websites or in an online institutional repository provided there appears always the proper citation of the Manuscript in MBoC and a link to the original publication of the Manuscript in MBoC.” See http://www.mbcpapers.org/html/MBC_Copyright.pdf
ISSN:	1059-1524
Last Modified:	20 May 2023 18:30
URI:	https://orca.cardiff.ac.uk/id/eprint/71

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