Hallett, Maurice Bartlett  ORCID: https://orcid.org/0000-0001-8197-834X and Petit, E. J.
1997.
The use of single image random dot stereograms for presenting 3D microscopic confocal images.
Journal of Microscopy
186
(3)
, pp. 275-278.
10.1046/j.1365-2818.1997.2130769.x
|
Abstract
Presenting 3D images produced by confocal optical slicing techniques in a static and easily publishable form can be difficult. Here, we demonstrate the presentation of the data as a single image random dot stereogram (SIRD), which can be viewed as a 3D object by ‘defocusing’ the eyes. The production of the SIRD employs three steps: (i) acquisition of the optical slices using confocal techniques, (ii) allocation of a suitable grey level to the object in each slice to provide depth-encoding information for the final image and (iii) calculation of the SIRD from the composite depth-encoded image. The technique is demonstrated with a limited number of optical slices through an acridine-orange-stained neutrophil (diameter =10 μm), in order to show the relative positions of the nuclear lobes in the cell.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine |
| Uncontrolled Keywords: | Confocal microscopy,stereogram |
| Publisher: | Wiley |
| ISSN: | 0022-2720 |
| Last Modified: | 28 Oct 2022 08:56 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/72671 |
Citation Data
Cited 1 time in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Dimensions
Dimensions