Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X and Petit, E. J. 1997. The use of single image random dot stereograms for presenting 3D microscopic confocal images. Journal of Microscopy 186 (3) , pp. 275-278. 10.1046/j.1365-2818.1997.2130769.x |
Abstract
Presenting 3D images produced by confocal optical slicing techniques in a static and easily publishable form can be difficult. Here, we demonstrate the presentation of the data as a single image random dot stereogram (SIRD), which can be viewed as a 3D object by ‘defocusing’ the eyes. The production of the SIRD employs three steps: (i) acquisition of the optical slices using confocal techniques, (ii) allocation of a suitable grey level to the object in each slice to provide depth-encoding information for the final image and (iii) calculation of the SIRD from the composite depth-encoded image. The technique is demonstrated with a limited number of optical slices through an acridine-orange-stained neutrophil (diameter =10 μm), in order to show the relative positions of the nuclear lobes in the cell.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine |
Uncontrolled Keywords: | Confocal microscopy,stereogram |
Publisher: | Wiley |
ISSN: | 0022-2720 |
Last Modified: | 28 Oct 2022 08:56 |
URI: | https://orca.cardiff.ac.uk/id/eprint/72671 |
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