The production of an amine-modified derivative of 5-aminosalicylic acid by activated neutrophils Roles for myeloperoxidase and chloride ions

Laffafian, I., Brown, R. C. and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 1991. The production of an amine-modified derivative of 5-aminosalicylic acid by activated neutrophils Roles for myeloperoxidase and chloride ions. Biochemical Pharmacology 42 (10) , pp. 1869-1874. 10.1016/0006-2952(91)90583-Q

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Abstract

Following incubation with activated neutrophils, two metabolites of 5-aminosalicylic acid (5-ASA) were identified by HPLC. These two metabolites accounted for approximately 60% and 20% of the original 5-ASA. The formation of the major metabolite was prevented by pre-incubation with the peroxidase inhibitor, azide, and reduced by the omission of chloride ions from the incubation medium, or the presence of catalase. A similar product was generated by sodium hypochlorite or myeloperoxidase/H2O2, mass spectroscopical analysis being consistent with it being 5-nitroso-salicylate. Our finding suggests that the efficacy of 5-ASA results from its ability to react with and so scavenge hypochlorite ions. The amount of amine-modified 5-ASA in the faecal stream may thus provide an indicator for hypochlorite production in the bowel.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Elsevier
ISSN:	0006-2952
Last Modified:	28 Oct 2022 09:01
URI:	https://orca.cardiff.ac.uk/id/eprint/72996

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