Jones, J., Hallett, Maurice Bartlett  ORCID: https://orcid.org/0000-0001-8197-834X and Morgan, B. P.
1990.
Reversible cell damage by T-cell perforins. Calcium influx and propidium iodide uptake into K562 cells in the absence of lysis.
Biochemical Journal
267
(2)
, pp. 303-307.
|
Abstract
The non-lethal effects of the lymphocyte-derived pore-forming toxin perforin on the human erythroleukaemia cell line K562 were investigated. By using the fluorescent Ca2+ indicator fura-2, perforin was shown to cause intracellular Ca2+ concentration to rise transiently into the micromolar range in the absence of cell death. By fluorescence-activated cell sorting it was demonstrated that K562 cells took up the membrane-impermeant nuclear stain propidium iodide (PI) when exposed to non-lethal doses of perforin. The permeability to PI was short-lived, confirming the transience of the perforin pore. Analogies with non-lethal effects and recovery processes occurring in nucleated cells exposed to the membrane-attack complex of complement are drawn
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Publisher: | Biochemical Society |
| ISSN: | 0264-6021 |
| Last Modified: | 28 Oct 2022 09:04 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/73171 |
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