Laffafian, Iraj and Hallett, Maurice Bartlett ![]() |
Abstract
Cytosolic free Ca2+ concentration was measured and imaged in human neutrophils moving towards a source of formylated peptide in a micropipette held close to the cells. Under these conditions, neutrophils changed shape and displayed chemotaxis without significant or persistent global or localised elevations in cytosolic free Ca2+. A rear-to-front persistent Ca2+ gradient of less than 0.5 nM/micron was present in the migrating neutrophils, until they reached the zone of higher peptide concentration, when an abrupt rise in cytosolic free Ca2+ concentration was triggered and chemotaxis stopped. Small localised rises in cytosolic free Ca2+, which were occasionally observed during neutrophil manoeuvring, were attributed to the effect of local deformation of the neutrophil membrane, since deformation of the membrane with a blunt micropipette caused similar Ca2+ changes. These data suggest that neutrophil chemotaxis towards a source of formylated peptide occurs without significant changes in Ca2+ signalling.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | R Medicine > R Medicine (General) |
Uncontrolled Keywords: | Calcium/physiology*, Cell Movement, Chemotactic Factors/metabolism, Chemotaxis, Humans, Image Processing, Computer-Assisted, Neutrophils/physiology* |
Publisher: | The Company of Biologists Ltd |
ISSN: | 0021-9533 |
Last Modified: | 28 Oct 2022 09:51 |
URI: | https://orca.cardiff.ac.uk/id/eprint/75899 |
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