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Purification and characterization of human and mouse complement C3

Ruseva, M.M. and Heurich-Sevcenco, Meike 2014. Purification and characterization of human and mouse complement C3. In: Gadjeva, Mihaela ed. The Complement System: Methods and Protocols, Vol. 1100. Methods in Molecular Biology, Humana Press, pp. 75-91. (10.1007/978-1-62703-724-2_6)

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Complement component C3 is the most abundant complement protein in plasma, central to all three complement activation pathways and essential to complement amplification. Thus, it is one of the most extensively studied complement proteins. This chapter describes the purification of C3 from human and mouse plasma using protein precipitation, followed by classical ion exchange chromatography and gel filtration. The biochemical and functional characteristics of the purified C3 are typically assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hemolysis assays. The hemolysis assay is a standard technique to assess complement activity monitoring the lysis of red blood cells

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Humana Press
ISBN: 9781627037235
ISSN: 1064-3745
Last Modified: 06 Mar 2020 15:47

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