Fluorescent in situ hybridization

Dyer, Sara A. and Green, Elaine Karen 2002. Fluorescent in situ hybridization. Theophilus, Bimal D. M. and Rapley, Ralph, eds. PCR Mutation Detection Protocols, Vol. 187. Methods in Molecular Biology, Humana Press, pp. 73-86. (10.1385/1-59259-273-2:073)

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Abstract

Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Humana Press
ISBN:	9780896036178
ISSN:	1064-3745
Last Modified:	06 Mar 2020 16:04
URI:	https://orca.cardiff.ac.uk/id/eprint/82542

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