Annulus of collagen fibrils in mouse cornea and structural matrix alterations in a murine-specific keratopathy

Quantock, Andrew James ORCID: https://orcid.org/0000-0002-2484-3120, Hayes, Sally ORCID: https://orcid.org/0000-0001-8550-0108, Adachi, Wakako, Kinoshita, Shigeru, Boote, Craig ORCID: https://orcid.org/0000-0003-0348-6547, Meek, Keith Michael Andrew ORCID: https://orcid.org/0000-0002-9948-7538, Matsushima, Yoshibumi and Tachibana, Masayoshi 2003. Annulus of collagen fibrils in mouse cornea and structural matrix alterations in a murine-specific keratopathy. Investigative Ophthalmology & Visual Science 44 (5) , pp. 1906-1911. 10.1167/iovs.02-0884

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Abstract

PURPOSE. Mouse corneas were investigated to see whether a limbal annulus of corneal collagen exists as in humans. Mice with corneas predisposed to topographical changes (the SKC strain) were also examined, to establish the size and spacing of stromal collagen fibrils and the integrity of the annulus. METHODS. X-ray diffraction was used to measure collagen fibril spacing and diameter in normal (the BALB/c strain; four male, two female) and SKC (six male and six female) corneas and to identify the degree of preferred collagen orientation at 200-µm intervals across two BALB/c and four SKC corneas. RESULTS. The average collagen fibril diameter measured 35.5 nm in 3-month-old BALB/c corneas, and 36.9 nm and 37.0 nm, respectively, in corneas of age-matched male and female SKC mice. In male and female SKC corneas, average collagen interfibrillar Bragg spacing was significantly higher (64.5 and 59.9 nm, respectively) than in corneas of BALB/c mice (49.7 nm). Circumferentially aligned collagen, indicative of a limbal annulus of fibrillar collagen 2.2 mm in diameter, was identified in mouse cornea. On occasion, this was disturbed in the SKC phenotype. CONCLUSIONS. Collagen fibrils are marginally larger in the corneas of SKC mice than in the corneas of BALB/c mice and are considerably more widely spaced. An annulus of fibrillar collagen probably exists near the limbus of the normal mouse cornea that may help promote biomechanical stability and maintain corneal shape. A loss of structural integrity in the annulus of some SKC mice may predispose the corneas to biomechanical instability and shape changes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Additional Information:	Confirmation received by publisher on 21 February 2014 that publisher's pdf can be self-archived 6 months after publication.
Publisher:	Association for Research in Vision and Ophthalmology
ISSN:	0146-0404
Date of First Compliant Deposit:	30 March 2016
Last Modified:	17 May 2023 16:56
URI:	https://orca.cardiff.ac.uk/id/eprint/840

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