Quantitative measurement of fathead minnow vitellogenin mRNA using hybridization protection assays

Thomas-Jones, E, Walkley, Neal, Morris, C., Kille, Peter ORCID: https://orcid.org/0000-0001-6023-5221, Cryer, J., Weeks, I. and Woodhead, J. S. 2003. Quantitative measurement of fathead minnow vitellogenin mRNA using hybridization protection assays. Environmental Toxicology and Chemistry 22 (5) , pp. 992-995. 10.1002/etc.5620220506

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Abstract

We have developed a novel test system for the quantitative assessment of gene transcription. The procedure involves the use of chemiluminescent-labeled oligonucleotide probes in a hybridization protection assay (HPA) format. We have used this technology to measure changes in vitellogenin mRNA to demonstrate the impact of estrogen exposure in the juvenile fathead minnow (Pimephales promelas). Marked changes in mRNA expression were observed in response to intraperitoneal injection of 17β-estradiol demonstrating the utility of this technique for the identification and monitoring of toxic responses to xenobiotics.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics
Publisher:	Society of Environmental Toxicology and Chemistry Press
ISSN:	0730-7268
Last Modified:	31 Oct 2022 10:21
URI:	https://orca.cardiff.ac.uk/id/eprint/84515

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