Microbial profiling of dental plaque from mechanically ventilated patients

Sands, Kirsty M., Twigg, Joshua A. ORCID: https://orcid.org/0000-0002-4567-7854, Lewis, Michael A. O. ORCID: https://orcid.org/0000-0003-1917-0651, Wise, Matt P., Marchesi, Julian R. ORCID: https://orcid.org/0000-0002-7994-5239, Smith, Ann, Wilson, Melanie ORCID: https://orcid.org/0000-0002-8834-8268 and Williams, David W. ORCID: https://orcid.org/0000-0002-7351-5131 2016. Microbial profiling of dental plaque from mechanically ventilated patients. Journal of Medical Microbiology 65 (2) , pp. 147-159. 10.1099/jmm.0.000212

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Abstract

Microorganisms isolated from the oral cavity may translocate to the lower airways during mechanical ventilation leading to ventilator-associated pneumonia (VAP). Changes within the dental plaque microbiome during mechanical ventilation (MV) have been documented previously, primarily using culture-based techniques. The aim of this study was to use community profiling by high throughput sequencing to comprehensively analyse suggested microbial changes within dental plaque during MV. 16S rDNA gene sequences were obtained from 38 samples of dental plaque sampled from 13 mechanically ventilated patients and sequenced using the Illumina platform. Sequences were processed using Mothur, applying a 97% gene similarity cut-off for bacterial species level identifications. A significant 'microbial shift' occurred in the microbial community of dental plaque during MV for 9 out of 13 patients. Following extubation, or removal of the endotracheal tube (ETT) that facilitates ventilation, sampling revealed a decrease in the relative abundance of potential respiratory pathogens and a compositional change towards a more predominantly (in terms of abundance) oral microbiota including Prevotella spp., and streptococci. The results highlight the need to better understand microbial shifts in the oral microbiome in the development of strategies to reduce VAP, and may have implications for the development of other forms of pneumonia such as community acquired infection.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Biosciences
Subjects:	R Medicine > RK Dentistry
Additional Information:	PDF uploaded in accordance with publisher's policies at http://www.sherpa.ac.uk/romeo/issn/0022-2615/ (accessed 18.2.16).
Publisher:	Society for General Microbiology
ISSN:	0022-2615
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	17 December 2015
Last Modified:	12 May 2023 19:23
URI:	https://orca.cardiff.ac.uk/id/eprint/84545

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