Synthesis of ROS scavenging microspheres from a dopamine containing poly(beta-amino ester) for applications for neurodegenerative disorders

Newland, Benjamin ORCID: https://orcid.org/0000-0002-5214-2604, Wolff, Paul, Zhou, Dezhong, Wang, Wei, Zhang, Hong, Rosser, Anne Elizabeth ORCID: https://orcid.org/0000-0002-4716-4753, Wang, Wenxin and Werner, Carsten 2016. Synthesis of ROS scavenging microspheres from a dopamine containing poly(beta-amino ester) for applications for neurodegenerative disorders. Biomaterials Science 4 (3) , pp. 400-404. 10.1039/C5BM00542F

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Abstract

Parkinson's disease (PD) is a common neurodegenerative disease characterized by a substantial decrease of dopaminergic neurons in the substantia nigra pars compacta. The neurological deterioration during PD can be, in part, attributed to elevated levels of reactive oxygen species (ROS). Radical scavengers have previously been shown to protect dopaminergic cells from toxic effects in vitro. Hence, new approaches need to be investigated to improve the administration of antioxidants in order to provide neuroprotection. Polymers exhibiting catechol structures offer one such approach due to their interesting physicochemical properties. In the present study a photocrosslinkable dopamine-containing poly(β-amino ester) (DPAE) was synthesized from poly(ethylene glycol) diacrylate (PEGDA) and dopamine hydrochloride using Michael type addition. A water-in-oil emulsion technique was used to photo-crosslink the polymer into spherical microparticles. DPAE microspheres featured excellent scavenging properties towards 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radicals in a dose dependent manner and could even reduce the dissolved oxygen content of physiological solution. Furthermore, the concentrations required for radical scavenging were shown to be non-toxic towards dopaminergic SH-SY5Y cells as well as primary astrocytes and primary embryonic rat ventral midbrain cultures.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) Medicine Neuroscience and Mental Health Research Institute (NMHRI) Pharmacy
Subjects:	R Medicine > R Medicine (General)
Additional Information:	PDF uploaded in accordance with publisher's policies at http://www.sherpa.ac.uk/romeo/issn/2047-4830/ (accessed 11.02.16).
Publisher:	Royal Society of Chemistry
ISSN:	2047-4830
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	23 December 2015
Last Modified:	09 May 2023 04:12
URI:	https://orca.cardiff.ac.uk/id/eprint/85330

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