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Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments

O'Sullivan, Louise Anne, Webster, Gordon ORCID:, Fry, John Christopher, Parkes, Ronald John and Weightman, Andrew ORCID: 2008. Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments. Journal of Microbiological Methods 75 (3) , pp. 579-581. 10.1016/j.mimet.2008.08.006

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Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by ≈23%.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Earth and Environmental Sciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: PCR-DGGE; 16S rRNA genes; Prokaryotic diversity; Bacteria Archaea
Publisher: Elsevier
ISSN: 0167-7012
Last Modified: 02 Dec 2022 11:45

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