Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas

Kille, Peter ORCID: https://orcid.org/0000-0001-6023-5221, Morgan, Andrew, Kate, Powell, Frederick, Mosselmans, Daniel, Hart, Paul, Gunning, Hayes, Anthony Joseph, Scarborough, Derek, McDonald, Iain ORCID: https://orcid.org/0000-0001-9066-7244 and John, Charnock 2016. Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas. Open Biology Journal 6 (3) , -. 10.1098/rsob.150270

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Abstract

Woodlice efficiently sequester copper (Cu) in ‘cuprosomes' within hepatopancreatic ‘S' cells. Binuclear ‘B’ cells in the hepatopancreas form iron (Fe) deposits; these cells apparently undergo an apocrine secretory diurnal cycle linked to nocturnal feeding. Synchrotron-based µ-focus X-ray spectroscopy undertaken on thin sections was used to characterize the ligands binding Cu and Fe in S and B cells of Oniscus asellus (Isopoda). Main findings were: (i) morphometry confirmed a diurnal B-cell apocrine cycle; (ii) X-ray fluorescence (XRF) mapping indicated that Cu was co-distributed with sulfur (mainly in S cells), and Fe was co-distributed with phosphate (mainly in B cells); (iii) XRF mapping revealed an intimate morphological relationship between the basal regions of adjacent S and B cells; (iv) molecular modelling and Fourier transform analyses indicated that Cu in the reduced Cu+ state is mainly coordinated to thiol-rich ligands (Cu–S bond length 2.3 Å) in both cell types, while Fe in the oxidized Fe3+ state is predominantly oxygen coordinated (estimated Fe–O bond length of approx. 2 Å), with an outer shell of Fe scatterers at approximately 3.05 Å; and (v) no significant differences occur in Cu or Fe speciation at key nodes in the apocrine cycle. Findings imply that S and B cells form integrated unit-pairs; a functional role for secretions from these cellular units in the digestion of recalcitrant dietary components is hypothesized.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Earth and Environmental Sciences Biosciences
Publisher:	Bentham Open / Bentham open
ISSN:	1874-1967
Funders:	STFC
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	9 February 2016
Last Modified:	20 May 2023 07:30
URI:	https://orca.cardiff.ac.uk/id/eprint/86957

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