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Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas

Kille, Peter, Morgan, Andrew, Kate, Powell, Frederick, Mosselmans, Daniel, Hart, Paul, Gunning, Hayes, Anthony Joseph, Scarborough, Derek, McDonald, Iain and John, Charnock 2016. Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas. Open Biology Journal 6 (3) , -. 10.1098/rsob.150270

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Woodlice efficiently sequester copper (Cu) in ‘cuprosomes' within hepatopancreatic ‘S' cells. Binuclear ‘B’ cells in the hepatopancreas form iron (Fe) deposits; these cells apparently undergo an apocrine secretory diurnal cycle linked to nocturnal feeding. Synchrotron-based µ-focus X-ray spectroscopy undertaken on thin sections was used to characterize the ligands binding Cu and Fe in S and B cells of Oniscus asellus (Isopoda). Main findings were: (i) morphometry confirmed a diurnal B-cell apocrine cycle; (ii) X-ray fluorescence (XRF) mapping indicated that Cu was co-distributed with sulfur (mainly in S cells), and Fe was co-distributed with phosphate (mainly in B cells); (iii) XRF mapping revealed an intimate morphological relationship between the basal regions of adjacent S and B cells; (iv) molecular modelling and Fourier transform analyses indicated that Cu in the reduced Cu+ state is mainly coordinated to thiol-rich ligands (Cu–S bond length 2.3 Å) in both cell types, while Fe in the oxidized Fe3+ state is predominantly oxygen coordinated (estimated Fe–O bond length of approx. 2 Å), with an outer shell of Fe scatterers at approximately 3.05 Å; and (v) no significant differences occur in Cu or Fe speciation at key nodes in the apocrine cycle. Findings imply that S and B cells form integrated unit-pairs; a functional role for secretions from these cellular units in the digestion of recalcitrant dietary components is hypothesized.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Earth and Environmental Sciences
Publisher: Bentham Open / Bentham open
ISSN: 1874-1967
Funders: STFC
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 9 February 2016
Last Modified: 20 Mar 2021 02:26

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