Assessing the impact of long term frozen storage of faecal samples on protein concentration and protease activity

Morris, Laura S. and Marchesi, Julian Roberto ORCID: https://orcid.org/0000-0002-7994-5239 2016. Assessing the impact of long term frozen storage of faecal samples on protein concentration and protease activity. Journal of Microbiological Methods 123 , pp. 31-38. 10.1016/j.mimet.2016.02.001

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Abstract

The proteome is the second axis of the microbiome:host interactome and proteases are a significant aspect in this interaction. They interact with a large variety of host proteins and structures and in many situations are implicated in pathogenesis. Furthermore faecal samples are commonly collected and stored frozen so they can be analysed at a later date. So we were interested to know whether long term storage affected the integrity of proteases and total protein and whether historical native faecal samples were still a viable option for answering research questions around the functional proteome.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	Gut; Microbiota; Proteases; Proteins; Long-term storage; Enzyme activity
Publisher:	Elsevier
ISSN:	0167-7012
Funders:	Biotechnology and Biological Sciences Research Council
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	3 July 2015
Last Modified:	11 Oct 2023 17:34
URI:	https://orca.cardiff.ac.uk/id/eprint/87076

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