The misfolded pro-inflammatory protein S100A9 disrupts memory via neurochemical remodelling instigating an Alzheimer's disease-like cognitive deficit

Gruden, Marina A., Davydova, Tatiana V., Wang, Chao, Narkevich, Victor B., Fomina, Valentina G., Kudrin, Vladimir S., Morozova-Roche, Ludmilla A. and Sewell, Robert David Edmund 2016. The misfolded pro-inflammatory protein S100A9 disrupts memory via neurochemical remodelling instigating an Alzheimer's disease-like cognitive deficit. Behavioural Brain Research 306 , pp. 106-116. 10.1016/j.bbr.2016.03.016

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Abstract

Memory deficits may develop from a variety of neuropathologies including Alzheimer’s disease dementia. During neurodegenerative conditions there are contributory factors such as neuroinflammation and amyloidogenesis involved in memory impairment. In the present study, dual properties of S100A9 protein as a pro-inflammatory and amyloidogenic agent were explored in the passive avoidance memory task along with neurochemical assays in the prefrontal cortex and hippocampus of aged mice. S100A9 oligomers and fibrils were generated in vitro and verified by AFM, Thioflavin T and A11 antibody binding. Native S100A9 as well as S100A9 oligomers and fibrils or their combination were administered intranasally over 14 days followed by behavioral and neurochemical analysis. Both oligomers and fibrils evoked amnestic activity which correlated with disrupted prefrontal cortical and hippocampal dopaminergic neurochemistry. The oligomer-fibril combination produced similar but weaker neurochemistry to the fibrils administered alone but without passive avoidance amnesia. Native S100A9 did not modify memory task performance even though it generated a general and consistent decrease in monoamine levels (DA, 5-HT and NA) and increased metabolic marker ratios of DA and 5-HT turnover (DOPAC/DA, HVA/DA and 5-HIAA) in the prefrontal cortex. These results provide insight into a novel pathogenetic mechanism underlying amnesia in a fear-aggravated memory task based on amyloidogenesis of a pro-inflammatory factor leading to disrupted brain neurochemistry in the aged brain. The data further suggests that amyloid species of S100A9 create deleterious effects principally on the dopaminergic system and this novel finding might be potentially exploited during dementia management through a neuroprotective strategy.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Additional Information:	Available online 8 March 2016 PDF uploaded in accordance with publisher's policies at http://www.sherpa.ac.uk/romeo/issn/0166-4328/ (accessed 1.4.16). This journal has an embargo period of 18 months
Publisher:	Elsevier
ISSN:	0166-4328
Date of First Compliant Deposit:	1 April 2016
Date of Acceptance:	5 March 2016
Last Modified:	05 Nov 2024 20:15
URI:	https://orca.cardiff.ac.uk/id/eprint/88524

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