Hybridization relics complicate barcode-based dentification of species in earthworms

Dupont, L., Porco, D., Symondson, William Oliver Christian ORCID: https://orcid.org/0000-0002-3343-4679 and Roy, V. 2016. Hybridization relics complicate barcode-based dentification of species in earthworms. Molecular Ecology Resources 16 (4) , pp. 883-894. 10.1111/1755-0998.12517

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Abstract

ntrogressive hybridization results in mito-nuclear discordance which could obscure the delimitation of closely related taxa. Although such events are increasingly reported, they have been poorly studied in earthworms. Here, we propose a method for investigating the degree of introgressive hybridization between three taxa of the Allolobophora chlorotica aggregate within two field populations (N = 67 and N = 105) using a reference data set including published DNA barcoding and microsatellite data of all known A. chlorotica lineages (N = 85). For this, we used both molecular phylogenetic and population genetic approaches. The test of correspondence between mitochondrial cytochrome c oxidase I (COI) lineages and clusters of nuclear microsatellite genotypes allowed individuals to be sorted in three categories (matching, admixed and nonmatching) and additional markers (mitochondrial NADH dehydrogenase subunit 1, nuclear Histone 3 and Internal transcribed Spacer Region 2) were used for phylogenetic reconstructions in order to check assignments. Although 15 admixed individuals were observed, no early-generation hybrids were detected within the two populations. Interestingly, 14 nonmatching individuals (i.e. with a mtDNA haplotype that did not correspond to their nuclear cluster) were detected, a pattern that would result after multiple generations of unidirectional hybridization of female from one taxon to male of the other taxon. Because earthworms are simultaneous hermaphrodites, these events of unidirectional hybridization suggest sterility of the male function in several crosses and highlight that some individuals can be misidentified if reliance is placed on COI barcodes alone. These findings could improve the use of these barcodes in earthworms for species delineation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QL Zoology
Uncontrolled Keywords:	cyto-nuclear discordance; DNA barcoding; earthworms; introgressive hybridization; molecular phylogenetics; population genetics
Publisher:	Wiley
ISSN:	1755-098X
Date of Acceptance:	5 February 2016
Last Modified:	01 Nov 2022 09:37
URI:	https://orca.cardiff.ac.uk/id/eprint/88653

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