Cell-cell adhesion molecules and signaling intermediates and their role in the invasive potential of prostate cancer cells.

Davies, G., Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 and Mason, Malcolm David ORCID: https://orcid.org/0000-0003-1505-2869 2000. Cell-cell adhesion molecules and signaling intermediates and their role in the invasive potential of prostate cancer cells. Journal of Urology 163 (3) , pp. 985-992.

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Abstract

Purpose: The highly variable natural history of prostate carcinoma may be reflected in heterogeneity of invasive potential between tumors. Materials and Methods: We have examined two prostate cancer cell lines of low invasive potential (CAHPV10 and PZHPV7) and three cell lines of high invasive potential (DU-145, PC-3, LNCapFGC), to determine whether specific adhesion molecule profiles correlated with their invasive behavior. Results: Using an in vitro invasion assay, we demonstrated that DU-145, LNCapFGC and PC-3 cells were highly invasive compared with CA-HPV-10 and PZ-HPV-7 cells. LNCapFGC cells expressed high levels of E-cadherin, α-, β- and γ-catenin, desmoglein, desmoplakin and GSK3β using immunoblotting. This was, in general, comparable to immunohistochemical staining. PC-3 cells had no E-cadherin or α-catenin, but expressed a high level of the HGF/SF receptor c-Met. In contrast, DU-145 cells were found to express E-cadherin and low levels for all other protein molecules, except c-Met. The DU-145 cell line also lacked α-catenin expression. In CA-HPV-10 and PZ-HPV-7 cells, there was no detection of APC, PECAM-1, P-cadherin or Wnt-1. DU-145, LNCapFGC and PC-3 cells formed cell-cell aggregates, which were reduced by inclusion of anti-E-cadherin antibody and the motogen HGF/SF. Conclusion: These results show that prostate cancer cells exhibit a diverse expression of cell-cell adhesion molecules and their signaling intermediates. The expression of these adhesion molecules bears an important relationship with the invasive phenotype of these cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Publisher:	American Urological Association
Last Modified:	01 Nov 2022 09:40
URI:	https://orca.cardiff.ac.uk/id/eprint/88803

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