Cadmium-induced genomic instability in Arabidopsis: molecular toxicological biomarkers for early diagnosis of cadmium stress

Wang, Hetong, He, Lei, Song, Jie, Cui, Weina, Zhang, Yanzhao, Jia, Chunyun, Francis, Dennis, Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857, Sun, Lizong, Tai, Peidong, Hui, Xiujuan, Yang, Yuesuo and Liu, Wan 2016. Cadmium-induced genomic instability in Arabidopsis: molecular toxicological biomarkers for early diagnosis of cadmium stress. Chemosphere 150 , pp. 258-265. 10.1016/j.chemosphere.2016.02.042

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Abstract

Microsatellite instability (MSI) analysis, random-amplified polymorphic DNA (RAPD), and methylation-sensitive arbitrarily primed PCR (MSAP-PCR) are methods to evaluate the toxicity of environmental pollutants in stress-treated plants and human cancer cells. Here, we evaluate these techniques to screen for genetic and epigenetic alterations of Arabidopsis plantlets exposed to 0–5.0 mg L−1 cadmium (Cd) for 15 d. There was a substantial increase in RAPD polymorphism of 24.5, and in genomic methylation polymorphism of 30.5–34.5 at CpG and of 14.5–20 at CHG sites under Cd stress of 5.0 mg L−1 by RAPD and of 0.25–5.0 mg L−1 by MSAP-PCR, respectively. However, only a tiny increase of 1.5 loci by RAPD occurred under Cd stress of 4.0 mg L−1, and an additional high dose (8.0 mg L−1) resulted in one repeat by MSI analysis. MSAP-PCR detected the most significant epigenetic modifications in plantlets exposed to Cd stress, and the patterns of hypermethylation and polymorphisms were consistent with inverted U-shaped dose responses. The presence of genomic methylation polymorphism in Cd-treated seedlings, prior to the onset of RAPD polymorphism, MSI and obvious growth effects, suggests that these altered DNA methylation loci are the most sensitive biomarkers for early diagnosis and risk assessment of genotoxic effects of Cd pollution in ecotoxicology.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Uncontrolled Keywords:	Microsatellite instability; Random-amplified polymorphic DNA; Methylation-sensitive arbitrarily primed PCR; Cadmium; Arabidopsis thaliana
Publisher:	Elsevier
ISSN:	0045-6535
Date of First Compliant Deposit:	7 April 2016
Date of Acceptance:	9 February 2016
Last Modified:	18 Nov 2024 10:00
URI:	https://orca.cardiff.ac.uk/id/eprint/88951

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