The clinical significance and impact of interleukin 15 on keratinocyte cell growth and migration

Jones, A. M., Griffiths, J. L., Sanders, Andrew J. ORCID: https://orcid.org/0000-0002-7997-5286, Owen, Sioned, Ruge, Fiona, Harding, Keith and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2016. The clinical significance and impact of interleukin 15 on keratinocyte cell growth and migration. International Journal of Molecular Medicine 38 , pp. 679-686. 10.3892/ijmm.2016.2687

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Abstract

Chronic wounds represent a significant burden to health services and are associated with patient morbidity. Novel methods to diagnose and/or treat problematic wounds are needed. Interleukin (IL)-15 is a cytokine involved in a number of biological processes and disease states such as inflammation, healing and cancer progression. The current study explores the expression profile of IL-15 and IL-15 receptor α (IL-15Rα) in chronic wounds and its impact on keratinocytes. IL-15 and IL-15Rα expression were examined in healing and non-healing chronic wounds using qPCR and immunohistochemical analysis. The impact of recombinant IL-15 (rhIL-15) on human adult low calcium temperature (HaCaT) keratinocyte growth and migratory potential was further examined. IL-15 transcript expression was slightly, though non-significantly elevated in healing chronic wounds compared with non-healing chronic wounds. IL-15 protein staining was minimal in both subtypes of chronic wounds. By contrast, IL-15Rα transcript and protein expression were both observed to be enhanced in non-healing chronic wounds compared with healing chronic wounds. The treatment of HaCaT cells with rhIL-15 generally enhanced cell growth and promoted migration. Analysis with small molecule inhibitors suggested that the pro-migratory effect of rhIL-15 may be associated with ERK, AKT, PLCγ and FAK signalling. IL-15 may promote healing traits in keratinocytes and the differential expression of IL-15Rα is observed in chronic wounds. Together, this may imply a complex role for this interleukin in wound healing.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Spandidos Publications
Date of First Compliant Deposit:	18 April 2016
Date of Acceptance:	15 April 2016
Last Modified:	07 May 2023 23:17
URI:	https://orca.cardiff.ac.uk/id/eprint/89367

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