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Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B

Wu, Xiaoyun, Zhou, You ORCID:, Zhang, Ke, Liu, Qingzhen and Guo, Deyin 2008. Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B. FEBS Letters 582 (15) , pp. 2155-2160. 10.1016/j.febslet.2008.05.033

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Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0014-5793
Date of Acceptance: 7 May 2008
Last Modified: 01 Nov 2022 10:00

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