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Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation

Harwood, Adrian John ORCID: 2008. Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation. Vincan, Elizabeth, ed. Wnt Signaling, Vol. 469. Methods in Molecular Biology, Springer, pp. 39-43. (10.1007/978-1-60327-469-2_4)

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GSK-3 activity mediates cAMP repression of stalk induction of cells in low-density monolayer culture. The lower the GSK-3 activity the greater the percentage of stalk cells formed. This protocol describes a robust and quantitative method utilizing an adapted stalk cell monolayer assay to measure GSK-3 activation.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Biosciences
Neuroscience and Mental Health Research Institute (NMHRI)
Subjects: Q Science > QR Microbiology
Publisher: Springer
ISBN: 9781603274685
ISSN: 1064-3745
Last Modified: 18 Oct 2022 12:17

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