Lymphangiogenesis quantification using quantitative PCR and breast cancer as a model

Cunnick, G.H., Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111, Gomez, K.F. and Mansel, Robert E. ORCID: https://orcid.org/0000-0002-8051-0726 2001. Lymphangiogenesis quantification using quantitative PCR and breast cancer as a model. Biochemical and Biophysical Research Communications 288 (4) , pp. 1043-1046. 10.1006/bbrc.2001.5869

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Abstract

The detection of lymphangiogenesis (formation of new lymphatics) has previously been difficult to measure, primarily due to the lack of specific markers for lymphatic endothelium. Using conventional PCR (polymerase chain reaction), DNA sequencing, plasmid synthesis, and real-time quantitative PCR (RTQPCR), we report a new approach to enable the measurement of lymphangiogenesis using LYVE-1, a novel, specific lymphatic marker in breast cancer tissue. By using a Scorpion-based probe system with the RTQPCR analyser, a highly sensitive and specific detection and quantitation of LYVE-1 was possible. It was found that lymphangiogenesis occurred in all breast specimens and that higher levels were found in tumours which had spread to the lymph nodes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Publisher:	Elsevier
ISSN:	0006-291X
Last Modified:	01 Nov 2022 10:40
URI:	https://orca.cardiff.ac.uk/id/eprint/92497

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