Comparison of plasma β-globin DNA and S-100 protein concentrations in acute stroke

Rainer, Timothy Hudson ORCID: https://orcid.org/0000-0003-3355-3237, Wong, Ka Sing, Lam, Wynnie, Lam, Nicole Y.L., Graham, Colin A. and Lo, Y.M. Dennis 2007. Comparison of plasma β-globin DNA and S-100 protein concentrations in acute stroke. Clinica Chimica Acta 376 (1-2) , pp. 190-196. 10.1016/j.cca.2006.08.025

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Abstract

Background This study aimed to compare changes in plasma β-globin DNA and serum S100 protein to diagnose stroke and for predicting mortality and morbidity. Methods Patients with stroke-like symptoms presenting to the emergency department of a Hong Kong hospital were recruited. Plasma DNA was analyzed for the β-globin gene with fluorescent-based PCR. S100 concentrations were determined using ELISA. Primary outcomes were diagnosis of stroke, mortality, and modified Rankin Score (mRS) after 6 months. Results One hundred ninety-seven consecutive patients recruited, 118 (60%) ischemic stroke, 35 (18%) hemorrhage and 44 (22%) with no acute neuroimaging changes. Serum S100 and plasma DNA were increased in 126 (p < 0.0010) and 36 (p = 0.21) stroke patients respectively vs. controls. Median plasma DNA was higher in hemorrhagic stroke than those without (1725 vs. 1050 kilogenome-equivalents/l, p = 0.0104). Median plasma DNA was higher in mRS > 2 vs. mRS ≤ 2 (1350 vs. 1025, p = 0.0103), and higher in non-survivors vs. survivors (1625 vs. 1050, p = 0.0070). Median serum S100 higher in mRS > 2 patients vs. mRS ≤ 2 (0.152 vs. 0.131 μg/l, p = 0.0003). The odds ratio (OR) of discriminating hemorrhagic from non-hemorrhagic stroke with DNA was 4.24 (95% CI 1.88–9.56); S100 and DNA together give an OR of 16.55. Conclusion For stroke diagnosis, S100 performs better than DNA; DNA is a better marker for hemorrhage. For diagnosis of hemorrhagic stroke, combined S100 and DNA performs better than either alone. Plasma DNA and serum S100 predict morbidity and mortality in stroke.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Uncontrolled Keywords:	Plasma β-globin DNA; Prognosis; S100 protein; Stroke; Emergency department
Publisher:	Elsevier
ISSN:	0009-8981
Date of Acceptance:	17 August 2006
Last Modified:	01 Nov 2022 10:46
URI:	https://orca.cardiff.ac.uk/id/eprint/92847

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