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Molecular characterisation of tumour infiltrating lymphocytes in oral squamous cell carcinoma

Stephens, Mark, Lim, Kenneth, Stephens, Philip ORCID:, Lim, S. H. and Thomas, David William ORCID: 1998. Molecular characterisation of tumour infiltrating lymphocytes in oral squamous cell carcinoma. Cancer Immunology, Immunotherapy 46 (1) , pp. 34-40. 10.1007/s002620050457

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Oral squamous cell carcinoma (OSCC) is often associated with a lymphocytic infiltrate that is believed to represent an in vivo immune reaction to the tumour cells. In this study, the tumour-infiltrating lymphocytes (TIL) associated with primary OSCC were characterised molecularly in six newly-diagnosed patients to determine the nature of the immune response to the primary tumour. The primary tumours in three of the six patients were associated with a moderate to dense CD8+ T cell infiltrate whilst the cellular infiltrate in the other primary tumours was sparse. The CD3+ T cells were also HLA-DR+. In all cases, there were few CD56+ cells, suggesting that TIL were predominantly T cells bearing the α/β T cell receptors (TCR). The TCR Vβ repertoire of TIL in these six cases was analysed. TCR Vβ gene usage by TIL was heterogeneous. A restricted usage of TCR Vβ genes by TIL was evident in two tumours associated with a dense CD3+ T cell infiltrate. In one of these, there was histological evidence of tumour cell destruction by TIL. Further analysis of the predominant TCR Vβ gene family used by TIL in this individual showed a unique in-frame nucleotide sequence in 100% of the transcripts. This recurrent transcript was not detected in the peripheral blood of this patient, indicating a local T cell clonal expansion in the vicinity of the tumour. Overall, these results suggest that activation and clonal expansion of T cells occurs and may play a role in local tumour destruction in OSCC.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Publisher: Springer
ISSN: 0340-7004
Last Modified: 02 Nov 2022 09:51

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