Griffiths, Peter Charles, Nilmini, Renuka, Carter, Emma  ORCID: https://orcid.org/0000-0001-6691-2377, Dodds, Patrick ORCID: https://orcid.org/0000-0001-9326-765X, Murphy, Damien Martin ORCID: https://orcid.org/0000-0002-5941-4879, Khayat, Zeena, Lattanzio, Ettore, Ferruti, Paolo, Heenan, Richard K., King, Stephen M. and Duncan, Ruth
2010.
Interaction of an endosomolytic polyamidoamine ISA23 with vesicles mimicking intracellular membranes: A SANS/EPR study.
Macromolecular Bioscience
10
(8)
, pp. 963-973.
10.1002/mabi.201000040
|
Abstract
The mechanism of ISA23 · HCl interaction with model membrane vesicles (80–100 nm in diameter) was investigated using EPR in conjunction with SANS. For EPR, 16-DSE was dissolved in the vesicle membrane to measure its dynamics and polarity, whereas a spin-labeled (Tempo)-ISA 23 analogue was used to give a measure of the polymer flexibility. When ISA23 was added to the external vesicle surface, no interaction was found. This observation conflicts with the reported ability to lyse RBC, but is in agreement with recent studies that showed no effect on membrane permeability when a PAA was added to an incubation medium containing isolated lysosomal vesicles. The vesicle-mimetic models used here provide a new and useful tool for studying endosomolytic polymer/membrane interactions.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Chemistry Pharmacy |
| Subjects: | Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica |
| Uncontrolled Keywords: | Electron spin resonance ; Endosomolytic polymers ; Membranes ; Polymer/surface interactions ; Spin labels |
| Publisher: | Wiley-Blackwell |
| ISSN: | 1616-5187 |
| Funders: | EPSRC |
| Last Modified: | 02 Dec 2022 12:11 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/9746 |
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