The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer

Chen, Gang, Yu, Hefen, Satherley, Lucy, Zabkiewicz, Catherine, Resaul, Jeyna, Zhao, Huishan, Mu, Hu, Zhi, Xiuyi, He, Junqi, Ye, Lin ORCID: https://orcid.org/0000-0002-0303-2409 and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2017. The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancer. Oncology Reports 37 (5) , pp. 2695-2701. 10.3892/or.2017.5538

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Abstract

The downstream of tyrosine kinase 7 (DOK7) is an adaptor protein mediating signalling transduction between receptors and intracellular downstream molecules. Reduced expression of DOK7 has been observed in breast cancer. The present study aimed to investigate the role played by DOK7 in lung cancer. The expression of DOK7 at both mRNA and protein levels was evaluated in human lung cancer. A reduced expression of DOK7 transcripts was seen in lung cancers compared with normal lung tissues. Kaplan-Meier analyses showed that the reduced expression of DOK7 was associated with poorer overall survival and progression-free survival of patients with lung cancer. A further western blot analysis revealed a predominant expression of DOK7 isoform 1 (DOK7V1) in normal lung tissues, which was reduced in lung cancer. Forced overexpression of DOK7V1 in lung cancer cell lines, A549 and H3122 resulted in a decrease of in vitro cell proliferation and migration, while adhesion to extracellular matrix was enhanced following the expression. In conclusion, DOK7 was reduced in lung cancer and reduced DOK7 expression was associated with poorer survival. DOK7 isoform 1 plays an inhibitory role on the proliferation and migration of lung cancer cells in which Akt pathway may be involved.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Spandidos Publications
ISSN:	1021-335X
Date of First Compliant Deposit:	5 April 2017
Date of Acceptance:	1 March 2017
Last Modified:	19 Aug 2024 12:36
URI:	https://orca.cardiff.ac.uk/id/eprint/99645

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