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Exploring the roles of chromatin remodelers in regulating chromatin organisation and transcription in Dictyostelium discoideum

Robinson, Mark 2016. Exploring the roles of chromatin remodelers in regulating chromatin organisation and transcription in Dictyostelium discoideum. PhD Thesis, Cardiff University.
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Nucleosomes comprise the most basic repeating unit of chromatin and provide hubs for the regulation of DNA transcription, replication and repair. ATPase chromatin remodelling complexes establish nucleosome occupancy, positioning and structure in a dynamic fashion to allow fine-tuning of protein-DNA interactions. The ISWI and CHD families of remodelers possess the ability to sample DNA linker length between nucleosomes and space nucleosomes evenly. How these spacing remodelers combine their functions to maintain phasing of nucleosomal arrays, and re-organise these arrays during development remains poorly understood. Furthermore the relationship between nucleosomal array structure and transcriptional regulation is unclear. Dictyostelium discoideum provides a complex chromatin environment and remodeler repertoire, while retaining a compact genome and ease of genetic manipulation. Thus we have utilized this model to generate remodeler null mutants, and double mutants to observe phenotypic effects and interactions. We further compiled comprehensive nucleosome mapping and RNA sequencing data sets for all spacing remodelers in Dictyostelium. Bioinformatic analysis of these data provide novel insights into remodeler functions, and help to establish a paradigm to explain the relationship between remodeler-mediated chromatin organisation and transcriptional regulation.

Item Type: Thesis (PhD)
Status: Unpublished
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Uncontrolled Keywords: chromatin nucleosomes transcription MNase-seq RNA-seq
Date of First Compliant Deposit: 13 April 2017
Date of Acceptance: 13 April 2017
Last Modified: 11 Dec 2020 02:37

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