Cerebrospinal fluid complement system biomarkers in demyelinating disease

Zelek, Wioleta Milena, Fathalla, Dina, Morgan, Angharad ORCID: https://orcid.org/0000-0003-4075-7676, Touchard, Samuel, Loveless, Samantha ORCID: https://orcid.org/0000-0002-5124-4115, Tallantyre, Emma ORCID: https://orcid.org/0000-0002-3760-6634, Robertson, Neil P. ORCID: https://orcid.org/0000-0002-5409-4909 and Morgan, B. Paul ORCID: https://orcid.org/0000-0003-4075-7676 2020. Cerebrospinal fluid complement system biomarkers in demyelinating disease. Multiple Sclerosis 26 (14) , pp. 1929-1937. 10.1177/1352458519887905

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Abstract

Background: Multiple sclerosis (MS) can be difficult to differentiate from other demyelinating diseases, notably neuromyelitis optica spectrum disorder (NMOSD). We previously showed that NMOSD is distinguished from MS by plasma complement biomarkers. Objective: Here, we measure cerebrospinal fluid (CSF) complement proteins in MS, NMOSD and clinically isolated syndrome (CIS), a neurological episode that may presage MS, to test whether these distinguish NMOSD from MS and CIS. Materials and methods: CSF (53 MS, 17 CIS, 11 NMOSD, 35 controls) was obtained; complement proteins (C4, C3, C5, C9, C1, C1q, Factor B (FB)), regulators (Factor I (FI), Factor H (FH), FH-Related Proteins 1, 2 and 5 (FHR125), C1 Inhibitor (C1INH), Properdin) and activation products (terminal complement complex (TCC), iC3b) were quantified by ELISA and results expressed relative to CSF total protein (μg/mg). Results: Compared to control CSF, (1) levels of C4, C1INH and Properdin were elevated in MS; (2) TCC, iC3b, FI and FHR125 were increased in CIS; and (3) all complement biomarkers except TCC, FHR125, Properdin and C5 were higher in NMOSD CSF. A statistical model comprising six analytes (C3, C9, FB, C1q, FI, Properdin) plus age/gender optimally differentiated MS from NMOSD.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	SAGE Publications
ISSN:	1352-4585
Date of First Compliant Deposit:	11 November 2019
Date of Acceptance:	12 October 2019
Last Modified:	05 Nov 2024 17:30
URI:	https://orca.cardiff.ac.uk/id/eprint/126709

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